Saturday, 14 November 2015

Bacterial Isolates from Contact Lenses, Frames and Their Susceptibility to Disinfectants

Abstract Bacteriological assessments of contact lenses and frames were determined using standard bacteriological methods and the susceptibility of the bacterial isolates to Clear Care Cleaning Disinfecting Solution (CCCDS), Eye-Look Optical Lens Cleaner (ELOC) and Hydrogen peroxide (H2O2) were assayed using disc diffusion technique. Bacterial isolates from the contact lenses and frames were: Staphylococcus aureus, Streptococcus spp , Pseudomonas aeruginosa, Coagulase negative (CoN) Staphylococcus spp , Bacillus spp, Citrobacter freundii, Corynebacterium spp , Escherichia coli , Haemophilus influenzae, Micrococcus spp. and Aeromonas hydrophila . Only 33 (55.0 %) contact lenses and 41 (68.3 %) frames swabs showed positive growth and of the 41 (68.3 %) frames’ swabs with positive growth, 23 (38.3 %) showed growth of single bacterial isolate, 10 (16.7 %) showed growth of two bacterial isolates, while polybacterial growth was present in 7 (13.3 %). Only 25 (75.8 %) males’ and 16 (59.3 %) females’ frames swabs had bacteria growth, while contact lenses and frames from aged 21-30 yrs and 41-50 yrs had the highest and lowest numbers of bacteria colonization, respectively. The highest and lowest bacteria colonization of contact lenses and frames were from the farmers and civil servants, respectively. Bacillus spp BS-F13, BS-F57 and CoN-Staphylococcus spp CS-C1 were resistant to CCCDS, ELOC and H2O2. P. aeruginosa PA-C50 and A. hydrophila AH-C32 were resistant to both ELOC and H2O2. Only 2/18 (11.1%) and 5/18 (27.8%) of the Gram negative bacteria were resistant to ELOC and H2O2, respectively. The inhibitory zones obtained using CCCDS and ELOC ranged from 6.7±2.5mm to 12.8±0.5mm and 6.8±0.5mm to 11.3±0.8mm, respectively. Conclusively, this study has provided data on the bacterial isolates associated with contact lenses, frames and also showed the considerable variations in the antibacterial efficacy of contact lenses disinfection solutions.

Keywords Bacteria, Disinfectants, Contact Lenses, Frames, Susceptibility.

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